To estimate effect of single nucleotide polymorphisms (SNP) on the estimated breeding value of Hungarian Grey (HG) bulls and to find markers associated with horn colour. Genotypes 136 HG animals were determined on Geneseek high-density Bovine SNP 150K BeadChip?. A multi-locus mixed-model was applied for statistical analyses. Six SNPs were identified to be associated (-log10P>10) with green and white horn. These loci are located on chromosome 1, 3, 9, 18 and 25. Seven loci (on chromosome 1, 3, 6, 9, 10, 28) showed considerable association (-log10P>10) with the estimated breeding value. Analysis provides markers for further research of horn colour and supplies markers to achieve more effective selection work regarding estimated breeding value of HG.Analysis provides markers for further research of horn colour and supplies markers to achieve more effective selection work regarding estimated breeding value of HG. If fertilization does not occur within a specific period of time, the quality of unfertilized oocytes in the oviduct (in vivo aging) or in culture (in vitro aging) will deteriorate over time. Icariin (ICA), found in all species of Epimedium herbs, has strong antioxidant activity and is thought to exert anti-aging effects in vitro. We asked whether ICA protects oocytes against age-related changes in vitro. We analyzed the reactive oxygen species (ROS) levels and expression of antioxidant, maternal, and estrogen receptor genes, and along with spindle morphology, and the developmental competence and quality of embryos in the presence and absence of ICA. Treatment with 5 µM ICA (ICA-5) led to a significant reduction in ROS activity, but increased mRNA expression of glutathione and antioxidant genes (SOD1, SOD2, PRDX5, and NFE2L2), during aging in vitro. In addition, ICA-5 prevented defects in spindle formation and chromosomal alignment, and increased mRNA expression of cytoplasmic maturation factor genes (BMP15, CCNB1, MOS, and GDF9). It also prevented apoptosis, increased mRNA expression of anti-apoptotic genes (BCL2L1 and BIRC5), and reduced mRNA expression of pro-apoptotic genes (BAK1 and CASP3). Although the maturation and cleavage rates were similar in all groups, the total cell number per blastocyst and the percentage of apoptotic cells at the blastocyst stage were higher and lower, respectively, in the control and ICA-5 groups than in the aging group. ICA protects oocytes against damage during aging in vitro; therefore, it can be used to improve assisted reproductive technologies.ICA protects oocytes against damage during aging in vitro; therefore, it can be used to improve assisted reproductive technologies. The objectives of this study were to investigate the direct antioxidative effect of Hsp90 obtained from duck muscle. The interaction of Hsp90 with phospholipids and oxidized phospholipids was studied with surface plasmon resonance (SPR), and their further oxidation in the presence of Hsp90 was evaluated with TBARS assay. The scavenging effect on the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS) was measured, and the electron paramagnetic resonance spectroscopy (EPR) in combination with BMPO and PTIO was utilized to determine the abilities of Hsp90 in scavenging hydroxyl and PTIO radicals. SPR showed Hsp90 could bind with both phospholipids and oxidized phospholipids, and prevent their further oxidation by the TBARS assay. The DPPH and ABTS scavenging activity increased with Hsp90 concentration, and could reach 27% and 20% respectively at the protein concentration of 50 μM. The EPR spectra demonstrated Hsp90 could directly scavenge •OH and PTIO• radicals. This suggests that Hsp90, a natural antioxidant in meat, may play an important role in cellular defense against oxidative stress, and may have potential use in meat products.This suggests that Hsp90, a natural antioxidant in meat, may play an important role in cellular defense against oxidative stress, and may have potential use in meat products. Wheat bran (WB) was co-fermented with Aspergillus oryzae and phytase to see if co-fermentation improve wheat bran phosphorus and fiber utilization in Isa-brown layers. A total of 112 Isa brown layer were randomly divided into 7 treatments with 8 replicates per a treatment and 2 hens per a replicate. The treatment include basal diet (Control), basal diet supplemented with 250 unit/kg phytase (Control+Phy), diet with 10% WB (10% WB), diet with 5% WB and 250 unit/kg phytase (5% WB+Phy) diet with 10% WB and 250 unit/kg phytase (10% WB+Phy), diet with 5% PCFWH and 125 unit/kg phytase (5% PCFWH), and diet with 10% PCFWH (10% PCFWH). The intestinal microbial population, intestinal morphology, serum antioxidant enzyme activities, and excreta phosphorus content was assessed. In PCFWH, spore counts, protease activity, xylanase activity, and ferulic acid were 8.50 log/g DM, 190 unit/g DM, 120 unit/g DM, and 127 μg/g, respectively. Xylobiose and xylotriose were released in PCFWH, while they were not detectable in WB. Antioxidant capacity was also enhanced in PCFWH compare to WB. The 10% WB+Phy and 10% PCFWH groups produced higher egg mass, a but hens fed 5% WB+Phy consumed the lowest amount of feed intake. https://www.selleckchem.com/products/motolimod-vtx-2337.html Eggs from 10% PCFWH had better eggshell weight, eggshell strength, and eggshell thickness. Birds fed with 10% PCFWH also had higher serum superoxide dismutase (SOD) and catalase (CAT) activities. Compare to control, 10% PCFWH significantly reduced excreta phosphorus content. 10 % PCFWH in diet improved egg quality, antioxidant status, and excreta phosphorus content of laying hens.10 % PCFWH in diet improved egg quality, antioxidant status, and excreta phosphorus content of laying hens. The present study evaluated the preservation of ram semen at 0°C using soybean lecithin with a Tris-fructose extender. Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×108 sperm/mL, followed by cooling to 0°C in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination. The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0. |