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A portable surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA) reader with multiplexed detection was developed using an integrated LFIA reaction column. The proposed LFIA reader was designed to simultaneously detect multiple samples or samples with multiple biomarkers. https://www.selleckchem.com/products/gdc6036.html With the integrated LFIA reaction column, we achieved the specific detection of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), and prostate-specific antigen (PSA) with a detection limit of 0.01 ng/mL, which was three orders of magnitude lower than that of the visual signal. We also investigated the uniformity of channels based on an eight-channel integrated LFIA reaction column. The relative standard deviation values of the SERS intensity of the eight-channel for measuring the AFP, CEA, and PSA antigens at 1323 cm-1 were 13%, 4.8%, and 5%, respectively. We detected 45 clinical serum samples of the three antigens using the proposed portable SERS-based LFIA reader to further confirm its applicability to clinical samples. The SERS signals of the positive sera were higher than those of the negative sera and their thrice standard deviation. This result indicated the practicality of the developed integrated reaction column and the proposed portable and multiplexed Raman reader. This work provides a new high-sensitivity, multiplexed, and automated SERS-based LFIA detector for use in the point-of-care setting.In this work, a signal amplification competitive-type photoelectrochemical system comprised of bismuth sulfide/bismuth oxyiodide/zinc oxide (Bi2S3/BiOI/ZnO) nano-array as platform and Ag2S-modified aptamers probe DNA (p DNA@Ag2S) as competition content for rapid and sensitive detection of OTA in microfluidic devices. The BiOI nano-array was first growth on surfaces of ZnO by a simple electrodeposited method, which provided large specific surface area and high stability to solve distribution of sensing platform and loose of combination of sensing substrate. Then, the Bi2S3 could be in-situ growth by self-sacrificial part Bi3+ of BiOI to form heterojunction without destroying the structure of the nano-array. A strong photocurrent intensity was acquired by the Bi2S3/BiOI/ZnO modified onto indium tin oxide (ITO) electrode, due to its good matching cascade band-edge levels could improve efficient separation of photo-generated e-/h+ pairs. After immobilizing with the capture DNA (c DNA) and the sequential hybridization of p DNA@Ag2S, the photocurrent intensity reduced obviously because part photo-generated electron transformed to Ag2S rather than Bi2S3/BiOI/ZnO electrode. Subsequently, the photocurrent intensity increased evident when immobilized the target OTA, owing to the OTA could bind the p DNA@Ag2S to form the specific-complex that were released from the electrode surface. Under optimal conditions, the prepared PEC microfluidic sensor exhibited a linear concentration of OTA from 0.01 pg/mL to 200 ng/mL with a low detection limit of 0.0035 pg/mL (S/N = 3). Furthermore, it achieved high sensitivity, good specificity, and acceptable stability and further provided an efficient method for sensitive detection of other target mycotoxins in practical application.Monocytes and macrophages are the two major cell types involved in innate immunity. Exosomes act as signaling molecules to regulate cell-to-cell communication by releasing proteins, mRNAs, microRNAs (miRNAs), and long noncoding RNAs (lncRNAs). However, it is still unclear whether monocyte-derived exosomes are involved in the communication between monocytes and macrophages. In this study, we analyzed the differentially expressed lncRNA profiles in monocytes isolated from blood samples of healthy controls and acute lung injury (ALI) patients. We focused our study on investigating the signaling downstream of CLMAT3 (colorectal liver metastasis-associated transcript 3), a lncRNA that regulated proinflammatory cytokine genes. We revealed that CLMAT3 specifically targeted CtBP2 (C-terminal binding protein 2) and repressed its expression. Elevated CtBP2 acted as a coactivator to assemble a transcriptional complex with histone acetyltransferase p300 and NF-κB (nuclear factor κB) subunits. In vitro coculture and in vivo injection of ALI monocyte-derived exosomes increased the production of proinflammatory cytokines. Importantly, the administration of two CtBP2 inhibitors, NSC95397 and MTOB, could significantly reverse CtBP2-mediated transactivation. Collectively, our results support a model in which monocyte-derived exosomal CLMAT3 activates the CtBP2-p300-NF-κB complex to induce proinflammatory cytokines, thus contributing to the pathogenesis of ALI.Structures of power and inequality shape day-to-day life for individuals who are poor, imposing waiting in multiple forms and for a variety of services, including for healthcare (Andaya, 2018a; Auyero, 2012; Strathmann and Hay, 2009). Constraints, such as the age requirements for Medicare, losing employer-provided health insurance, or the bureaucracy involved in filing for disability often require people to wait to follow recommendations for medical treatments. In 2016-2017, we conducted 52 narrative interviews in St. Louis, a city with significant racial and economic health inequities and without Medicaid expansion. We interviewed people with one or more chronic illnesses for which they were prescribed medication and who identified as having difficulties affording their prescriptions. Throughout the interviews, participants frequently recounted 1) experiences of waiting for care, along with other services, and 2) the range of strategies they utilized to manage the waiting. In this article, we develop the concept of active waiting to describe both the lived experiences of waiting for care and the responses that people devise to navigate, shorten, or otherwise endure waiting. Waiting is structured into healthcare and other social services at various scales in ways that reinforce feelings of marginalization, and also that require work on the part of those who wait. While much medical and public health research focuses on issues of diagnostic or treatment delay, we conclude that this conceptualization of active waiting provides a far more productive frame for accurately understanding the emotional and physical experiences of individuals who are disproportionately poor and made to wait for their care. Only with such understanding can we hope to build more just and compassionate social systems.

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